Description
CIC C1q ELISA Assay Kit is based on the binding of C1q-linked immunocomplexes to C1q adsorbed on microplate. In the first step, the samples are added to the microplate adsorbed with C1q; during the following incubation, C1q-fixing circulating immune complexes (CIC) bind to the C1q immobilized on the microplate. The microplate is washed for remove the unbound serum proteins. In the second step, the anti-human IgG conjugated with horseradish peroxidase (HRP) is added; it binds to the immunocomplex fixed on the microplate. The washing step removes the unbound conjugate. In the third step, the TMB Substrate is added, and this reacts with the conjugate fixed on the microplate, developing a colorimetric reaction. The quantity or CIC IgG complex is proportional to the colour intensity read at 450 nm wavelengths. The immunocomplex concentration in the sample is calculated through a calibration cuve. Heat aggregate human gamma globulin per mL (µgEq/mL) is the unit of measure of the results